anti mouse igg 492 alkaline phosphatase ap (SouthernBiotech)

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SouthernBiotech anti mouse igg 492 alkaline phosphatase ap
Anti Mouse Igg 492 Alkaline Phosphatase Ap, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 94/100, based on 203 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+human+igg+ap/Goat+Anti-Mouse+IgG%2C+Human+ads-AP/pm41935836-277-7-16
Average 94 stars, based on 203 article reviews

anti mouse igg 492 alkaline phosphatase ap - by Bioz Stars, 2026-10

94/100 stars

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Recombinase Polymerase Amplification:

Article Title: Citrullination of glucokinase is linked to autoimmune diabetes
Article Snippet: .. 2) For human subjects, all transportation and experiments were done by investigators blinded to the group allocation. anti-glucokinase (Proteintech, 19666-1-AP, Lot # 00014819 and # 00023801) anti-peptidyl-citrulline (Millipore, MABN328, clone F95, Lot # 3130480) anti-citrulline antibody (Abcam, ab6464) Goat anti-Human IgG-AP, (SouthernBiotech, 2040-04) Goat anti-Mouse IgM (u chain specific) Horseradish Peroxidase conjugated (Sigma-Aldrich, AP128P) Goat anti-rabbit IgG (H+L) Alexa Fluor 647 conjugated (ThermoFisher, A21244) Goat anti Mouse IgM Alexa Fluor 488 conjugated (ThermoFisher, A21042) anti-CD4 BV650 conjugated (Biolegend, 317436, clone RPA-T4, Lot # B284308) anti-CD3 APC conjugated (eBiosciences, 17-0038-42, clone UCHT1, Lot # 2071259) anti-CD25 FITC conjugated (Biolegend, 302604, clone BC96, Lot # B253407) anti-CD4 V500 conjugated (BD Bioscience, 560768, clone RPA-T4, Lot # 8325744) anti-CD14 PerCP-Cy5.5 conjugated (eBiosciences, 45-0149-42, clone 61D3, Lot # 1933247) anti-CD19 PerCP-Cy5.5 conjugated (eBiosciences, 45-0198-42, clone SJ25C1, Lot # 4297194) anti-CD45RA AF700 conjugated (BD Bioscience, 560673, clone HI100, Lot # 7047628) anti-CXCR3 FITC conjugated (Biolegend, 353704, clone G025H7, Lot # B185804) anti-CCR6 BV421 conjugated (Biolegend, 353408,clone G034E3, Lot # B206002) 3 nature portfolio | rep orting sum m ary M arch 2021 Validation Eukaryotic cell lines Policy information about cell lines Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals Wild animals Field-collected samples Ethics oversight Note that full information on the approval of the study protocol must also be provided in the manuscript. .. Human research participants Policy information about studies involving human research participants Population characteristics Recruitment anti-CCR4 BV605 conjugated (Biolegend, 359418, clone L291H4, Lot # B265240) ViaProbe PerCP-Cy5.5 conjugated (BD Bioscience, 555815, Lot # 7146688) All commercially available antibodies used are validated.

Biomarker Discovery:

other:

Article Title: Thermostable bivalent & trivalent filovirus vaccines from insect cells: potency demonstrated after 3 months and 2 years.
Article Snippet: In brief, protein was transferred onto a nitrocellulose membrane using the Bolt Mini Blot Module (Thermo Fisher Scientific, Waltham, MA) and blocked in PBS with 4 % milk powder (Nestlé) for 1 h at room temperature before being stained with antigen-specific monoclonal antibodies (human or humanized recombinant antibodies 13C6 for EBOV GP, FVM04 for SUDV GP and 9A11 for MARV GP) in blocking buffer with 0.1 % Tween 20.

Article Title: Anti-nuclear antibody (ANA) detection in culture supernatant of cloned B cells
Article Snippet: Goat anti-human IgM-AP (cat# 2020-04, Southern Biotech), goat anti-human IgG-AP (cat# 2040-04, Southern Biotech), and goat anti-human IgA-AP (cat # 2050-04, Southern Biotech).

Incubation:

Article Title: Prophylactic Activity of Orally Administered FliD-Reactive Monoclonal SIgA Against Campylobacter Infection.
Article Snippet: .. In the case of SIgA, detection was performed using human pIgR biotinylated antibody biotinylated anti-human SC antibody (R&D System) followed by incubation with streptavidin-AP, while goat anti-human IgG-AP (SouthernBiotech) was used for IgG. .. SIgA purified from human milk (MP Biomedicals) and Rituximab (MabThera) were used as quantification standards for SIgA and IgG, respectively.

Enzyme-linked Immunosorbent Assay:

Article Title: Optimization of Anti-ADAMTS13 Antibodies for the Treatment of ADAMTS13-Related Bleeding Disorder in Patients Receiving Circulatory Assist Device Support
Article Snippet: Brie y, at-bottom 96-well microtiter plates (Thermo Fisher Scienti c, 167008) were coated with Recombinant Human ADAMTS13 (Full Length) Protein (R&D Systems, 6156-AD-020) at 50 ng/well, Page 8/18 incubated overnight at 4°C, and blocked with ELISA blocking buffer (1×phosphate-buffered saline (PBS), 2% bovine serum albumin (BSA), 0.05% NaN3) at room temperature for 1 h. Three-fold serial dilutions of the mAbs from 1 μg/mL to 0.001 μg/mL in ELISA diluent (1×PBS, 1% BSA, 0.05% NaN3) were incubated in triplicate on the protein-coated wells for 1~2 h. Chimeric A10 and mA10 were used as positive controls, and 8A7 and 16E8, and mouse IgG2b-UNLB (SouthernBiotech, 1090-01) were used as negative controls for human and mouse ELISA tests, respectively. .. After washing the ELISA plates, human or mouse mAbs bound to the coated ADAMTS13 protein were detected by Goat Anti-Human IgG-AP (SouthernBiotech, 2040-04) or Goat Anti-Mouse IgG-AP (SouthernBiotech, 1030-04) with an alkaline phosphatase substrate (Sigma-Aldrich, S0942), respectively. .. The absorbance at 405 nm (A405) and 650 nm (A650) was read using a Multimode Plate Reader Enspire instrument (PerkinElmer), and A650 was subtracted from A405.

Article Title: Optimization of anti-ADAMTS13 antibodies for the treatment of ADAMTS13-related bleeding disorder in patients receiving circulatory assist device support
Article Snippet: Briefly, flat-bottom 96-well microtiter plates (Thermo Fisher Scientific, 167,008) were coated with Recombinant Human ADAMTS13 (Full Length) Protein (R&D Systems, 6156-AD-020) at 50 ng/well, incubated overnight at 4°C, and blocked with ELISA blocking buffer (1 × phosphate-buffered saline (PBS), 2% bovine serum albumin (BSA), 0.05% NaN 3 ) at room temperature for 1 h. Three-fold serial dilutions of the mAbs from 1 μg/mL to 0.001 μg/mL in ELISA diluent (1 × PBS, 1% BSA, 0.05% NaN 3 ) were incubated in triplicate on the protein-coated wells for 1 ~ 2 h. Chimeric A10 and mA10 were used as positive controls, and 8A7 and 16E8, and mouse IgG2b-UNLB (SouthernBiotech, 1090–01) were used as negative controls for human and mouse ELISA tests, respectively. .. After washing the ELISA plates, human or mouse mAbs bound to the coated ADAMTS13 protein were detected by Goat Anti-Human IgG-AP (SouthernBiotech, 2040–04) or Goat Anti-Mouse IgG-AP (SouthernBiotech, 1030–04) with an alkaline phosphatase substrate (Sigma-Aldrich, S0942), respectively. .. The absorbance at 405 nm (A 405 ) and 650 nm (A 650 ) was read using a Multimode Plate Reader Enspire instrument (PerkinElmer), and A 650 was subtracted from A 405 .

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